TABLE 1.
ΔTm values obtained by differential scanning fluorimetry and thermodynamic parameters for titration of PsPto-PscA-LBD with acid amino acids and their amidesa
| Ligand | ΔTm (°C) | Mean KD
(μM) ± SEM |
Mean ΔH
(kcal/mol)± SEM |
|---|---|---|---|
| l-Asp | +12.88 | 1.2 ± 0.1 | −6.7 ± 0.1 |
| d-Asp | +11.63 | 1.2 ± 0.1 | −4.7 ± 0.1 |
| l-Asn | +0.31 | No binding | |
| d-Asn | +0.97 | No binding | |
| l-Glu | +12.35 | 3.4 ± 0.1 | −7.8 ± 0.1 |
| d-Glu | +0.42 | No binding | |
| l-Gln | +0.03 | No binding | |
| l-Gln | ND | No binding | |
| N-Phthaloyl-l-Glu | +6.23 | No binding |
a
Compounds that resulted in a >2°C shift in melting temperature are in boldface type. ND, not determined.