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. 2019 Sep 26;10:1082. doi: 10.3389/fpls.2019.01082

Figure 3.

Figure 3

qRT-PCR analysis of BR-related gene expression under melatonin treatment. Seedlings were grown in light for 4 days and then transferred to 1/2MS plates with various concentrations of melatonin in a dark condition for another 3 days. Expression levels were normalized to an internal control ACTIN2. (A) qRT-PCR analysis of BR-biosynthetic genes. (B) qRT-PCR analysis of BR-induced genes involved in cell elongation. (C) qRT-PCR analysis of the two key transcription factors, BZR1 and BES1. Data are represented as means ± SD (n = 3), *p < 0.05, **p < 0.01 (Student’s t test). BR, brassinosteroid; MS, Murashige and Skoog; qRT-PCR, real-time polymerase chain reaction.