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. 2019 Sep 26;10:1082. doi: 10.3389/fpls.2019.01082

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Copyright © 2019 Xiong, Zhuo, Reiter, Wang, Wei, Deng, Song, Qanmber, Feng, Yang, Li and Ren

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Melatonin reduces endogenous BR levels. Seedlings 12 DAG from 1/2MS amended with melatonin, BRZ, or DMSO (control) were collected for the determination of BR levels by ELISA method. (A) Phenotype of seedlings from melatonin, BRZ, or DMSO treatments. (B) Hypocotyl length of the corresponding seedlings. *p < 0.05, **p < 0.01 (Student’s t test). Error bars indicate the SEM (n ≥ 40). (C) Endogenous bioactive BR content. Data are represented as means ± SD (n = 3), *p < 0.05 (Student’s t test). BR, brassinosteroid; BRZ, brassinazole; DAG, days after germination; DMSO, dimethyl sulfoxide; ELISA, enzyme-linked immunosorbent assay; MS, Murashige and Skoog; SEM, standard error of the mean.