Skip to main content
. 2019 Sep 26;10:2188. doi: 10.3389/fmicb.2019.02188

Figure 3.

Figure 3

3HP-β-LG inhibited HPV PsV entry by targeting virus, not cells. (A) 3HP-β-LG was unable to inactivate HPV PsV. HPV PsV was incubated with 3HP-β-LG (control: β-LG) at room temperature for 2 h and then separated by PEG-8000 to analyze its entry activity. The DMEM-treated virus was taken as negative control. (B) 3HP-β-LG blocked HPV PsV entry into the target cell. HeLa cells were incubated with HPV PsV, while 3HP-β-LG (15 μg/ml) was added at different time points (−0.5, 0, 0.5, 1, 2, 4, 8, 12, 24, and 48 h) to analyze the inhibitory activity. (C) 3HP-β-LG blocked HPV PsV entry by targeting virus. Temperature shift assay for anti-HPV activity of 3HP-β-LG (control: β-LG). Diluted 3HP-β-LG or β-LG at 15 μg/ml was mixed with HPV PsV and prechilled at 4°C for 30 min; then they were added to HeLa cells for 12 h. After washing twice, the cells were further incubated at 37°C for 72 h and then analyzed for fluorescent integrated density. (D) 3HP-β-LG inhibited HPV PsV entry by targeting the virus, not the host cell. HeLa cells were incubated with 3HP-β-LG (control: β-LG) at 37°C for 1 h with/without washing by DMEM before addition of HPV PsV. Each sample was tested in triplicate, and the experiment was repeated twice. Data from a representative experiment are presented as mean ± SD. Asterisks represent significant differences. ***p < 0.001.