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. 2019 Sep 26;10:2188. doi: 10.3389/fmicb.2019.02188

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Copyright © 2019 Hua, Zhu, Wu, Si, Wang, Sui and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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3HP-β-LG could not enter into blood circulation when applied through the vagina or anus. (A) Concentration of 3HP-β-LG in sera of monkeys through vaginal administration. (B) Concentration of 3HP-β-LG in sera of monkeys through rectal administration. 3HP-β-LG was formulated in a carbomer gel (1.8 mg/each) and administered through the vagina or anus of rhesus macaques. Their blood samples were collected at different time points before and after the application (−1 to 24 h). 3HP-β-LG in the blood samples was analyzed by ELISA. Each sample was tested in triplicate, and the experiment was repeated twice. Data from a representative experiment are presented as mean ± SD. The asterisks represent significant differences: ^*p < 0.05; ^**p < 0.01; ^***p < 0.001.