Table 1.
Sequence information of the markers used for polymorphic studies and foreground screening.
| S. No. | Marker name | Forward sequence (5’-3’) | Reverse sequence (5’-3’) | Annealing |
|---|---|---|---|---|
| 1 | phi112 | TGCCCTGCAGGTTCACATTGAGT | AGGAGTACGCTTGGATGCTCTTC | 53°C |
| 2 | umc1066 | ATGGAGCACGTCATCTCAATGG | AGCAGCAGCAACGTCTATGACACT | 60°C |
| 3 | phi 057 | CTCATCAGTGCCGTCGTCCAT | CAGTCGCAAGAAACCGTTGCC | 63°C |
| 4 | crtRB1 | ACACCACATGGACAAGTTCG | ACACTCTGGCCCATGAACAC (R1) | 62°C -54°Ca |
| ACAGCAATACAGGGGACCAG (R2) | 54°C |
a
In total of 19 cycles, reduction of temperature by 0.5°C on each cycle starting from the initial 54–62°C.