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. 2019 Sep 26;10:2228. doi: 10.3389/fmicb.2019.02228

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Copyright © 2019 Li, Peng, Wang, Hua, Xing, Zheng, Liu, Sun and Wei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Putative effectors in Rhizoctonia solani induce cell death symptoms on Nicotiana benthamiana leaves. (A) One protein out of 13 tested putative effectors, i.e., AGLIP1, induced the cell death in N. benthamiana, while the other 12 proteins did not. GFP, which did not trigger cell death, was shown as a negative control. AvrBs2 in X. oryzae pv. oryzicola was shown as a positive control. Numbers, e.g., 16/30, indicate that 16 of 30 infiltrated leaves exhibited cell death phenotypes. Representative photos were taken 3 days after DEX treatment. (B) Transient expressions of 13 tested putative effectors, AvrBs2 and GFP in N. benthamiana were confirmed by Western blot assay. For AGLIP1, the sample for protein extraction was collected at 1 day after DEX treatment, while samples for other proteins extraction were collected at 3 days after DEX treatment. The proteins with 3 × HA tag were detected by immunoblotting with an anti-HA antibody.