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. 2019 Sep 26;10:2228. doi: 10.3389/fmicb.2019.02228

FIGURE 3.

FIGURE 3

Expression analyses of AGLIP1 during R. solani infection and subcellular localization of AGLIP1 in rice cell. (A) Expression analyses of AGLIP1 during R. solani infection in rice cv. Nipponbare. The R. solani-inoculated rice sheaths were collected at 0, 12, 24, 48, 72, and 96 h post-inoculation for gene expression analyses using quantitative real time reverse transcription-polymerase chain reaction assay. The expression level of gpd was used as an internal reference for normalizing within the samples. Data are means ± standard error. (B) Subcellular localization of AGLIP1-GFP transiently expressed in rice protoplasts. The vector pUC19 carrying GFP was used as a control. The overlapped fluorescence was observed in rice protoplasts when co-expressed with AGLIP1-GFP and HDEL-mCherry via laser scanning confocal microscopy. The photo was taken before cell death symptom was induced.