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. 2019 Aug 27;10(20):4913–4920. doi: 10.7150/jca.32582

Fig 2.

Fig 2

Establishment of SYT12 knockdown cells and decreased cell growth via cell cycle arrest at G1 phase. (A, B) Expression of SYT12 mRNA and protein in shMock and shSYT12 cells (HSC-3 and SAS-derived transfectants) (N=3). (C) The cellular growth of the shSYT12 cells are decreased significantly (P < 0.05) compared with the shMock cells after 7 days (168 h) (N=3). (D) A flow cytometric analysis shows that the percentage of the shSYT12 cells in the G1 phase is increased compared with the shMock cells (N=3). (E) Immunoblotting analysis shows up-regulation of p27Kip1 and down-regulation of cyclin E and CDK2 in the shSYT12 cells compared with the shMock cells (N=3).