Figure 1.

Fucoidan-Sargassum inhibits the proliferation of HCC cell lines SMMC-7721, Huh7 and HCCLM3 but not the normal liver cell line HL-7702. (A) The IC₅₀ was determined for the SMMC-7721, Huh7 and HCCLM3 cell lines using the cell viability assay after the cells were treated with the indicated concentrations of Fucoidan-Sargassum for 0 to 72 h. (B) SMMC-7721, Huh7, and HCCLM3 cells were treated for 0-72 h with 4 concentrations of Fucoidan-Sargassum that were under the IC₅₀ to examine the dose dependency and inhibition rate (refer to Table 1 for detail statistical information). (C) The normal liver cell line HL-7702 was tested for cytotoxicity with indicated concentrations of Fucoidan-Sargassum (no statistical significance).