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. 2019 Aug 27;10(20):4777–4792. doi: 10.7150/jca.26740

Figure 2.

Figure 2

Figure 2

Fucoidan-Sargassum inhibits migration and invasion of HCC, SMMC-7721, Huh7 and HCCLM3 cells in a dose-dependent manner. (A-C) A Transwell assay was performed to evaluate the impact of Fucoidan-Sargassum on the migratory and invasive capabilities of SMMC-7721, Huh7, and HCCLM3 cells. Before seeding the cells into the inner chamber, both SMMC-7721 and HCCLM3 cells were treated with Fucoidan-Sargassum (0, 10, 20, and 30 mg/mL), and Huh7 cells were treated with (0, 5, 10, and 20 mg/mL). After 48 h of incubation in the inner chamber, the migrated and invasive cells were photographed. Each HCC cell line is represented by two bar graphs showing the effectiveness of Fucoidan-Sargassum treatment on inhibiting the migration and invasion of the cells in a dose-dependent manner (*P < .05, **P < .01, ***P < .001, ****P < .0001 vs. control, by ANOVA). (D) The HCCLM3 cells were seeded onto six-well culture plates and grown to 90% confluence in culture medium containing 10% fetal bovine serum. The cells were scratched with a 10 µL pipette tip and subsequently treated with Fucoidan-Sargassum (0, 10, 20, and 30 mg/mL). The images are representative of the inhibitory effect of dose increments of Fucoidan-Sargassum on the migration of HCCLM3 cells (highly metastatic) (top lane; incubation for 0 h, bottom lane; incubation for 72 h) (*P < .05, **P < .01 vs. control, by ANOVA).