Figure 3.

CDK5 and p35/p25 protein expression in frontal cortex after exposure to TA: Protein levels were measured as stated in “Protein extraction and Western blotting” section. Mice (N = 7/group) were administered with vehicle (corn oil) or 5 mg/kg TA dissolved in corn oil for 34 consecutive days. Quantification of protein in the frontal cortex of mice aged 17–18 months normalized against GAPDH. P < 0.05 is considered significant via Student’s t-test, noted by “*”. All values are expressed as mean ± standard error of the mean. (a) Quantification of CDK5 normalized against GAPDH; each bar in the histogram is representative of mean densitometric value of four individual mice per group (n = 4). (NC-CTRL, NC-TA) p > 0.05. (C-CTRL, C-TA) p = 0.03. (b) Quantification of p35 normalized against GAPDH, n = 3, p > 0.05 for all groups. Quantification of p25 normalized against GAPDH, each bar in the histogram is representative of three independents analysis (n′ = 3). (NC-CTRL, NC-TA) p > 0.05. (C-CTRL, C-TA) p = 0.05.

NC: non-carriers for tau; TA: tolfenamic acid.