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. 2018 May 22;39(10):2048–2060. doi: 10.1177/0271678X18777916

Figure 2.

Figure 2.

Role of annexin A2 (ANXA2) in the regulation of trans-endothelial permeability after hypoxia plus IL-1β insult. (a) ANXA2 expression in ANXA2 (A2) and control (Con) short interfering RNA (siRNA) transfected human brain microvascular endothelial cell cultures. n = 6 independent experiments (*P < 0.05, unpaired Student's t-test). (b) Effect of ANXA2 (A2) and control (Con) siRNA on trans-endothelial permeability. n = 5 independent experiments (*P < 0.05, unpaired Student's t-test). (c) The effects of recombinant ANXA2 (rA2) dose range in 24 h exposure of IL-1β (20 ng/ml)-induced trans-endothelial permeability increase. n = 5 independent experiments (*P < 0.05 vs. IL-1β group, one-way analysis of variance (ANOVA) followed by Tukey's tests). (d) The effect of 100 nM rA2 in 24 h hypoxia-induced trans-endothelial permeability increase. n = 5 independent experiments (*P < 0.05 vs. control, #P < 0.05 vs. hypoxia group, one-way ANOVA followed by Tukey's tests). (e) rA2 (100 nM) effects in IL-1β (20 ng/ml) plus hypoxia-induced trans-endothelial permeability increase. (f, g) rA2 (100 nM) effects in IL-1β (20 ng/ml) plus hypoxia-induced (f) relative and (g) absolute trans-endothelial electrical resistance (TEER) decrease. n = 3 independent experiments (*P < 0.05 vs. control, #P < 0.05 vs. hypoxia + IL-1β group, one-way ANOVA followed by Tukey's tests).