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. 2019 Aug 28;10(21):5139–5152. doi: 10.7150/jca.30816

Fig 3.

Fig 3

The roles of NF90 in ESCC cell proliferation and migration are miR-548k dependent. (A) miR-548k expression in NF90 overexpressed and miR-548k simultaneously depleted and control KYSE30 cells was measured by qRT-PCR. (B) Glo cell viability assay was performed to detect cell viability of NF90 overexpressed and miR-548k simultaneously depleted and control KYSE30 cells. (C) EdU incorporation assay was performed to detect cell proliferation of NF90 overexpressed and miR-548k simultaneously depleted and control KYSE30 cells. The red color represents EdU-positive and proliferation active cells. Scale bars, 100 μm. (D) Transwell assay was performed to detect cell migration of NF90 overexpressed and miR-548k simultaneously depleted and control KYSE30 cells. Scale bars, 100 μm. Results are presented as mean ± S.D. (n = 3). **P < 0.01, ***P < 0.001, ns, not significant, by one-way ANOVA followed by Dunnett's multiple comparison test.