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. 2019 Aug 28;10(21):5139–5152. doi: 10.7150/jca.30816

Fig 4.

Fig 4

The positive feedback loop between NF90, miR-548k, and lncRNA-LET. (A) Schematic outline of the reciprocal regulation between NF90, miR-548k, and lncRNA-LET. Red arrowhead represents positive regulation, and while blue arrowhead represents negative regulation. (B) lncRNA-LET expression in NF90 overexpressed and control KYSE30 cells was measured by qRT-PCR. (C) lncRNA-LET expression in NF90 depleted and control Eca-109 cells was measured by qRT-PCR. (D) lncRNA-LET expression in NF90 overexpressed and miR-548k simultaneously depleted and control KYSE30 cells was measured by qRT-PCR. (E) lncRNA-LET expression in lncRNA-LET overexpressed and control Eca-109 cells was measured by qRT-PCR. (F) miR-548k and pri-miR-548k expressions in lncRNA-LET overexpressed and control Eca-109 cells were measured by qRT-PCR. (G) lncRNA-LET expression in lncRNA-LET depleted and control KYSE30 cells was measured by qRT-PCR. (H) miR-548k and pri-miR-548k expressions in lncRNA-LET depleted and control KYSE30 cells were measured by qRT-PCR. (I) miR-548k and pri-miR-548k expressions in lncRNA-LET and NF90 simultaneously overexpressed and control Eca-109 cells were measured by qRT-PCR. Results are presented as mean ± S.D. (n = 3). ***P < 0.001, ns, not significant, by Student's t-test (B, E and F) or one-way ANOVA followed by Dunnett's multiple comparison test (C, D, G, H and I).