Skip to main content
. 2019 Aug 28;10(21):5139–5152. doi: 10.7150/jca.30816

Fig 5.

Fig 5

The NF90/miR-548k/lncRNA-LET feedback loop regulates the expression of HIF-1α, VEGF, KLF10, EGFR, and p53. (A) Schematic outline of the regulation of HIF-1α, VEGF, KLF10, EGFR, and p53 by NF90, miR-548k, and lncRNA-LET. Red arrowhead represents positive regulation, and while blue arrowhead represents negative regulation. (B) HIF-1α, VEGF, KLF10, EGFR, and p53 expressions in NF90 overexpressed and control KYSE30 cells were measured by qRT-PCR. (C) HIF-1α, VEGF, KLF10, EGFR, and p53 expressions in NF90 depleted and control Eca-109 cells were measured by qRT-PCR. (D) miR-548k expression in miR-548k overexpressed and control KYSE30 cells was measured by qRT-PCR. (E) miR-548k expression in miR-548k depleted and control Eca-109 cells was measured by qRT-PCR. (F) HIF-1α, VEGF, KLF10, EGFR, and p53 expressions in miR-548k overexpressed and control KYSE30 cells were measured by qRT-PCR. (G) HIF-1α, VEGF, KLF10, EGFR, and p53 expressions in miR-548k depleted and control Eca-109 cells were measured by qRT-PCR. (H) HIF-1α, VEGF, KLF10, EGFR, and p53 expressions in lncRNA-LET overexpressed and control Eca-109 cells were measured by qRT-PCR. (I) HIF-1α, VEGF, KLF10, EGFR, and p53 expressions in lncRNA-LET depleted and control KYSE30 cells were measured by qRT-PCR. Results are presented as mean ± S.D. (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, by Student's t-test (B, D, E, F, G and H) or one-way ANOVA followed by Dunnett's multiple comparison test (C, I).