Fig 6.
Activation of the NF90/miR-548k/lncRNA-LET feedback loop significantly promotes ESCC progression. (A) NF90 expressions in NF90 and miR-548k simultaneously overexpressed and lncRNA-LET simultaneously depleted KYSE30 cells were measured by western blot. (B) miR-548k and lncRNA-LET expressions in NF90 and miR-548k simultaneously overexpressed and lncRNA-LET simultaneously depleted KYSE30 cells were measured by qRT-PCR. (C) Glo cell viability assay was performed to detect cell viability of NF90 and miR-548k simultaneously overexpressed and lncRNA-LET simultaneously depleted KYSE30 cells. (D) EdU incorporation assay was performed to detect cell proliferation of NF90 and miR-548k simultaneously overexpressed and lncRNA-LET simultaneously depleted KYSE30 cells. The red color represents EdU-positive and proliferation active cells. Scale bars, 100 μm. (E) Transwell assay was performed to detect cell migration of NF90 and miR-548k simultaneously overexpressed and lncRNA-LET simultaneously depleted KYSE30 cells. Scale bars, 100 μm. For A-E, results are presented as mean ± S.D. (n = 3). ***P < 0.001 by Student's t-test. (F) NF90 and miR-548k simultaneously overexpressed and lncRNA-LET simultaneously depleted KYSE30 cells were subcutaneously injected into nude mice. Xenograft tumor volumes were detected every four days. (G) Xenograft tumor weights were detected at the 20th days after injection. For F-G, results are presented as mean ± S.D. (n = 5 mice). **P < 0.01 by Mann-Whitney U test.