Fig 7.
Targeting the NF90/miR-548k/lncRNA-LET feedback loop significantly represses ESCC progression. (A) NF90 expressions in NF90 and miR-548k simultaneously depleted, and lncRNA-LET simultaneously overexpressed Eca-109 cells were measured by western blot. (B) miR-548k and lncRNA-LET expressions in NF90 and miR-548k simultaneously depleted, and lncRNA-LET simultaneously overexpressed Eca-109 cells were measured by qRT-PCR. (C) Glo cell viability assay was performed to detect cell viability of NF90 and miR-548k simultaneously depleted and lncRNA-LET simultaneously overexpressed Eca-109 cells. (D) EdU incorporation assay was performed to detect cell proliferation of NF90 and miR-548k simultaneously depleted and lncRNA-LET simultaneously overexpressed Eca-109 cells. The red color represents EdU-positive and proliferation active cells. Scale bars, 100 μm. (E) Transwell assay was performed to detect cell migration of NF90 and miR-548k simultaneously depleted and lncRNA-LET simultaneously overexpressed Eca-109 cells. Scale bars, 100 μm. For A-E, results are presented as mean ± S.D. (n = 3). ***P < 0.001 by Student's t-test. (F) NF90 and miR-548k simultaneously depleted and lncRNA-LET simultaneously overexpressed Eca-109 cells were subcutaneously injected into nude mice. Xenograft tumor volumes were detected every seven days. (G) Xenograft tumor weights were detected at the 28th days after injection. For F-G, results are presented as mean ± S.D. (n = 5 mice). **P < 0.01 by Mann-Whitney U test.