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. 2019 Aug 28;10(21):5114–5123. doi: 10.7150/jca.31232

Figure 3.

Figure 3

Sinularin induces apoptosis in 786-O cells. After treated with various doses of Sinularin (20, 40, 80μM) for 24 h. (A) Cell apoptosis was measured by flow cytometry. (B) Cellular lysates were subjected to western blot analysis with indicated antibodies. (C) Caspases activities were assayed. (D) Cells were treated with Sinularin alone (80 μM) or in combination with Z-DEVD-FMK or Z-VAD-FMK for 24 h and viability were measured. (E) Cells were treated with Sinularin alone (80 μM) or in combination with Z-DEVD-FMK or Z-VAD-FMK for 24 h and apoptosis were measured. The data are expressed as the mean±SD from three independent experiments.*P<0.05, **P<0.01, ***P<0.001.