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. 2019 Aug 28;10(21):5153–5161. doi: 10.7150/jca.29937

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General program for CD155Bi-Ab and analyze of ATC. (A) General program for the generation of CD155Bi-Ab. (B) The concentrations of CD155Bi-Ab were measured by Coomassie blue staining of SDS-gel. (C) The assay of CD155Bi-Ab by flow cytometry. (D) The molecule expression on ATC analyzed by flow cytometry. (E) The optimal concentration of CD155Bi-Ab armed ATC was measured. ATC was armed with CD155Bi-Ab ranging from 5 to 500 ng/10⁶ cells at E/T ratio of 10:1, and the cytotoxic effects of CD155Bi-armed ATC on T24/DDP-luc cells were detected. After one-night incubation of CD155 Bi-armed ATCs with T24/DDP-luc cells, the percentage of viability was calculated at different concentrations.