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. 2019 Aug 28;10(21):5244–5255. doi: 10.7150/jca.30143

Figure 2.

Figure 2

Effects of BrMC on stemness of H460 cells induced by pro-inflammatory cytokines. H460 CSCs after chronic exposure to TGF-β followed by TNF-α incitation were treated with BrMC. The inhibition of BrMC was tested by cancer sphere forming (A~E) and soft ager colony forming (F~J) with single CSCs suspension. The effection on stemness capacity was evaluated by expressions of lung stem cell markers CD133, CD44, Bmi1 and Oct4 via western blot (K). The regulation on activation of NF-κB signaling and proto-oncogene FoxM1 were analyzed by western blot in CSCs under different concentrations of BrMC (1, 5, 10 μM). *P<0.05, vs untreated cells; #P<0.05, vs treated with BrMC only.