Figure 4.

Effects of thiostrepton on inhibition of stemness of H460 cells induced by proinflammatory cytokines by BrMC. H460 cells by administered TNF-α following chronic exposure to TGF-β were treated with thiostrepton (THI, 10.0 μmol/L) or BrMC (5.0 μmol/L) or both. Representative formed sphere (A~D) and cell colony (F~I) show the CSCs stemness capacity, and bar graph (E, J) show the rate individually. In presence (+) or absence (-) of thiostrepton, expressions of stem cell markers CD133, CD44, Bmi1 and Oct4 were analyzed by western blot (K). Inhibition of BrMC on CSCs was determined together with (+) or without (-) THI. Involvement of NF-κBp65 signaling and proto-oncogene FoxM1 were evaluated by WB. ^*P<0.05, vs untreated cells. ^#P<0.05, vs treated cells with THI only.