Table 2.
Stability values and ranking results for reference genes using NIH-3T3 cells. Lower stability values indicate more stable gene expression.
| Used method and ranking | Ppia | B2m | Actb | Hsp90ab1 | Gusb | Tbp | Gapdh | Pgk1 | Hprt |
|---|---|---|---|---|---|---|---|---|---|
| Delta CT | 3.94 | 3.92 | 4.46 | 4.09 | 4.84 | 5.2 | 4.04 | 10.82 | 10.67 |
| Ranking | 2 | 1 | 5 | 4 | 6 | 7 | 3 | 9 | 8 |
| BestKeeper | 0.57 | 0.88 | 1.56 | 1.09 | 1.33 | 2.13 | 1.02 | 7.27 | 9.50 |
| Ranking | 1 | 2 | 6 | 4 | 5 | 7 | 3 | 8 | 9 |
| Normfinder | 0.21 | 0.12 | 0.49 | 0.28 | 0.16 | 0.55 | 0.18 | 0.77 | 0.56 |
| Ranking | 4 | 1 | 6 | 5 | 2 | 7 | 3 | 9 | 8 |
| GeNorm | 0.56 | 0.56 | 1.12 | 0.92 | 2.07 | 1.52 | 0.74 | 5.78 | 4.34 |
| Ranking | 1 | 1 | 5 | 4 | 7 | 6 | 3 | 9 | 8 |
| Ranking G.M.a | 1 | 2 | 6 | 5 | 4 | 7 | 3 | 9 | 8 |
a
G.M., geometric mean.