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. Author manuscript; available in PMC: 2019 Oct 3.
Published in final edited form as: J Virol Methods. 2017 Jun 26;248:100–106. doi: 10.1016/j.jviromet.2017.06.012

Table 3.

Stability values and ranking results of reference genes using different tissues. Lower stability values indicate more stable gene expression.

Lung Ppia B2m Actb Hsp90ab1 Gusb Tbp Gapdh Pgk1 Hprt Tubb Pthlh
Delta CT 2.50 1.34 1.11 1.71 1.09 1.08 1.82 1.32 1.11 1.64 1.87
Ranking 11 6 3 8 2 1 9 5 4 7 10
BestKeeper 2.02 1.80 1.61 2.49 1.63 1.70 1.22 1.44 1.49 2.46 1.26
Ranking 9 8 5 11 6 7 1 3 4 10 2
Normfinder 2.34 0.84 0.16 1.37 0.20 0.17 1.62 0.74 0.24 1.25 1.67
Ranking 11 6 1 8 3 2 9 5 4 7 10
GeNorm 1.51 0.57 0.31 1.01 0.40 0.38 1.18 0.66 0.31 0.87 1.29
Ranking 11 5 1 8 4 3 9 6 1 7 10
Ranking G.M.a 11 7 1 10 4 2 6 5 3 9 8
Salivary Gland Ppia B2m Actb Hsp90ab1 Gusb Tbp Gapdh Pgk1 Hprt Tubb Pthlh
Delta CT 0.93 1.36 0.99 0.76 0.72 0.77 0.84 1.05 1 0.74 1.53
Ranking 6 10 7 3 1 4 5 9 8 2 11
BestKeeper 0.90 1.35 0.56 0.88 0.64 0.62 0.42 1.20 1.22 0.59 1.03
Ranking 7 11 2 6 5 4 1 9 10 3 8
Normfinder 0.62 1.18 0.70 0.38 0.14 0.40 0.49 0.82 0.81 0.23 1.41
Ranking 6 10 7 3 1 4 5 9 8 2 11
GeNorm 0.54 0.85 0.72 0.44 0.30 0.30 0.40 0.66 0.59 0.36 0.97
Ranking 5 9 8 4 1 1 3 7 6 2 11
Ranking G.M.a 7 11 6 5 1 3 4 9 8 2 10
Spleen Ppia B2m Actb Hsp90ab1 Gusb Tbp Gapdh Pgk1 Hprt Tubb Pthlh
Delta CT 0.48 0.34 0.35 0.51 0.42 0.5 0.65 0.4 0.43 0.72 0.57
Ranking 6 1 2 8 4 7 10 3 5 11 9
BestKeeper 0.32 0.29 0.26 0.46 0.45 0.48 0.47 0.42 0.25 0.47 0.31
Ranking 5 3 2 8 7 11 10 6 1 9 4
Normfinder 0.34 0.03 0.03 0.37 0.24 0.36 0.58 0.19 0.23 0.66 0.46
Ranking 6 1 1 8 5 7 10 3 4 11 9
GeNorm 0.24 0.06 0.06 0.36 0.20 0.33 0.44 0.18 0.28 0.49 0.39
Ranking 5 1 1 8 4 7 10 3 6 11 9
Ranking G.M.a 6 2 1 9 5 8 10 4 3 11 7
a

G.M., geometric mean.