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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Food Chem Toxicol. 2019 Aug 29;133:110793. doi: 10.1016/j.fct.2019.110793

Figure 1. Dietary exposure to E 171 did not significantly alter systemic or gastrointestinal tract resident CD103+ dendritic cell frequency.

Figure 1.

The frequency of gut resident and circulating CD103+ DC was determined in rats fed dietary E 171 for 7 or 100 consecutive days. Rats in the 100 day study were pretreated with 180 mg/kg of DMH or Vh control by i.p. injection. Following the indicated feeding period, animals were euthanized and PBMC, spleen, and Peyer’s patches were collected and processed into single cell suspension. Cells were then stained for CD11b/c, CD103, and MHCII and analyzed by flow cytometry to quantify CD103+ DC populations. CD103+ DC from the 7 day study are shown in panel A and from the 100 day study are shown in panel B. Data are presented as a percentage of the live cells within the lymphocyte gate. Data were analyzed for statistical significance using a one-way ANOVA with a Dunnett’s multiple comparisons test when there were no significant differences in the variance of the standard deviation.