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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Food Chem Toxicol. 2019 Aug 29;133:110793. doi: 10.1016/j.fct.2019.110793

Figure 2. Dietary E 171 exposure did not change the frequency of CD4+ Thelper cells systemically or in intestinal Peyer’s patches.

Figure 2.

The frequency of gut resident and circulating total and activated Thelper cells were determined in rats fed dietary E 171 for 7 or 100 days. Rats in the 100 day study were pretreated with 180 mg/kg of DMH or Vh control by i.p. injection. After the feeding period, animals were euthanized and PBMC, spleen, and Peyer’s patches were collected and processed into single cell suspensions. Cells were then stained for CD4 and CD25 and analyzed by flow cytometry. Activated Thelper cells were identified as being CD4/CD25 double positive. Total Thelper from 7 (A) and 100 (C) day animals and activated Thelper from 7 (B) and 100 (D) day studies are shown. Data are presented as a percentage of the live cells in the lymphocyte gate staining positive. Data were analyzed for statistical significance using a one-way ANOVA with a Dunnett’s multiple comparisons test when there were no significant differences in the variance of the standard deviation.