Table 3.

The summary of McCabe’s methods (2015)

Steps	Dual Smad inhibition	→	Cornea media
Medium	DMEM/F12, 20%KSR, SB431542 (10 mM), NOGGIN (500 ng/ml), bFGF (8 ng/ml)		DMEM/F12, 20%KSR, PDGF-BB (10 ng/ml), DKK-2 (10 ng/ml), bFGF (8 ng/ml)
Coating	Matrigel-coated well		Matrigel-coated well
Duration	3 days		14 days