Table 1.

Criteria and techniques used to establish the presence of arboviral sylvatic cycles

Criteria	Techniques employed to demonstrate the criteria
Sufficient populations of immuno-naïve (susceptible) NHPs	Antibody detection
Sufficient viraemia in the NHP to infect feeding mosquitoes	Viral isolation from blood
	Viral genome detection from blood
	Antibody detection
Sufficient populations of spatially and temporally coincident competent (capable of viral transmission) infected mosquitoes that feed on NHPs	Mosquito capture and identification
	Virus detection in mosquitoes
	Blood-meal analysis of fed mosquitoes
	Observations of NHPs being fed upon by forest mosquitoes
	Viral transmission experiments (between NHPs and mosquitoes)