Figure 6.
Effects of hutZEp mutation on resistance to serum and hutZEp expression under serum tolerance. A Survival of E. piscicida in fish serum. TX01, TX01ΔhutZ, and TX01ΔhutZC were incubated with non-immune flounder serum or PBS (control). After incubation, the survival of the bacteria was determined by plate counting. B RT-qPCR was performed with total RNA extracted from cultured Edwardsiella piscicida incubated in LB medium and incubated in flounder serum. Data are presented as the means ± SEMs (N = 3). N, the number of times the experiment was performed. *P < 0.05; **P < 0.01.