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. 2019 Oct 2;50:76. doi: 10.1186/s13567-019-0693-4

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of hutZ_Ep mutation on biofilm growth. A Biofilm-forming capacity of E. piscicida. TX01 and TX01ΔhutZ were incubated in polystyrene plates, and biofilm formation was determined by measuring the A₅₇₀ of the final eluates. B The viability of biofilm growth of E. piscicida as determined by confocal laser scanning microscopy (CLSM). Cells in the biofilms were stained with a BacLight LIVE/DEAD kit to reveal viable (green fluorescence) and non-viable (red fluorescence) bacteria. Data are presented as the means ± SEMs (N = 3). N, the number of times the experiment was performed. **P < 0.01.