Table 7.
Expression of miRNAs in the miR-17-92 cluster and paralogues according to molecular subtype, using PCR
| MiRNA | Molecular subtype | Mean (SD) | Contrasts | p* |
|---|---|---|---|---|
| miR-17-5p | Luminal A | − 5.49 (0.73) | Luminal A vs others | 0.066 |
| Luminal B | − 4.93 (1.43) | Luminal B vs others | 0.65 | |
| HER2+ | − 5.03 (1.70) | HER2+ vs others | 0.58 | |
| Triple-negative | − 3.26 (0.95) | Triple-negative vs others | 0.0025 | |
| miR-20a-5p | Luminal A | 1.72 (0.68) | Luminal A vs others | 0.066 |
| Luminal B | 2.21 (1.18) | Luminal B vs others | 0.67 | |
| HER2+ | 2.15 (1.19) | HER2+ vs others | 0.65 | |
| Triple-negative | 3.45 (1.03) | Triple-negative vs others | 0.0053 | |
| miR-92a-3p | Luminal A | 1.44 (0.74) | Luminal A vs others | 0.070 |
| Luminal B | 1.75 (1.00) | Luminal B vs others | 0.58 | |
| HER2+ | 1.89 (0.97) | HER2+ vs others | 0.85 | |
| Triple-negative | 2.87 (0.90) | Triple-negative vs others | 0.0073 | |
| miR-106b-5p | Luminal A | − 0.014 (0.55) | Luminal A vs others | 0.12 |
| Luminal B | 0.30 (1.11) | Luminal B vs others | 0.65 | |
| HER2+ | 0.54 (1.44) | HER2+ vs others | 0.91 | |
| Triple-negative | 1.10 (0.84) | Triple-negative vs others | 0.070 | |
| miR-93-5p | Luminal A | 1.20 (0.73) | Luminal A vs others | 0.070 |
| Luminal B | 1.78 (0.97) | Luminal B vs others | 0.93 | |
| HER2+ | 1.85 (1.21) | HER2+ vs others | 0.91 | |
| Triple-negative | 2.37 (1.03) | Triple-negative vs others | 0.085 |
Expression levels are presented as mean (standard deviation) of normalized PCR quantification cycle (Cq) values based on the average of the normalizer assay detected in all samples and the formula: normalized Cq = average Cq (all samples) − assay Cq (sample). Negative PCR expression values indicate lower expression levels compared to the normalizer
* False discovery rate (FDR) adjusted p-value for contrast tests comparing miRNA expression levels in one molecular subtype of cancers to the other cancers grouped together. E indicates exponential number