Fig. 6.

lnc-DILC enhances the interaction between PTEN and USP11. a Biotinylated lnc-DILC or antisense lnc-DILC (AS-lnc-DILC) were incubated with ccRCC cell extracts, targeted with streptavidin beads, and associated proteins were resolved in a gel. Western blotting analysis of the specific association of USP11 and lnc-DILC. Antisense lncDILC was shown as a negative control. b RIP experiments were performed to examine the RNA associated with USP11. GAPDH mRNA was taken as negative control. c ACHN and 786-O cells cotransfected with pcDNC3.1-lnc-DILC and USP11 siRNAs were treated with MG132 for 4 h before harvest. PTEN was immunoprecipitated and immunoblotted with the indicated antibodies. d The effect of lnc-DILC overexpression on the interaction between PTEN and USP11 was determined by co-immunoprecipitation assay in ACHN and 786-O cells. e The effect of lnc-DILC knockdown on the interaction between PTEN and USP11 was determined by co-immunoprecipitation assay in Caki-1 cells. **p < 0.01, ***p < 0.001