Figure 2.

Silencing AZGP1 prevents colorectal cancer metastasis by inhibiting the EMT in vitro and in vivo. (A) Western Blot assay the efficiency of lentivirus of shRNA at AZGP1. (B) MTT assay the proliferation of colorectal cancer cell when silencing AZGP1. (C) Transwell migration assay was performed on HCT116 and SW480 cells when silencing AZGP1. Representative images are shown. (D-G) A liver metastasis model was used to examine metastasis in vivo. The weights of mice were monitored during the mold. After the appearance of cachexia, mice were sacrificed, representative liver with metastasis (D), representative H&E staining of a liver with nodules, liver metastases ratio (F) and the liver to body weight ratio (G) were determined. (H) Western blot analysis of EMT markers. (I) Representative immunofluorescence images of EMT markers N-cadherin and Vimentin. Short hairpin RNA (shRNA) targeting the human AZGP1 gene was designed as follows: sense 5'-TGGTTGTGAGATCGAGAATAA-3'.