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. 2019 Aug 29;10(22):5557–5566. doi: 10.7150/jca.35380

Figure 4.

Figure 4

PAK2 mediated phosphorylation of FLNA involved in the promotion metastasis by AZGP1. KEGG pathway analysis from microassay. (B) Western blot analysis of proteins involved in the adhesion pathway. And the general expression and phosphorylation of FLNA when AZGP1 was down-regulated. (C)Western blot analysis of proteins PAK2, RhoA, Rac1/2/3. (D) Transwell assay the migration after over-expressed AZGP1 and treated with PAK2 inhibitor FRAX597. (E)Western blot analysis the marker of EMT and phosphorylation of FLNA after over-expressed AZGP1 and treated with PAK2 inhibitor FRAX597 (1uM, 48h). (F)Distribution of FLNA, a conductor protein of AZGP1, in the cytoplasm and nucleus.(G) Proposed mechanism by which AZGP1 promotes metastasis: when AZGP1 is highly expressed, it is inclined to interact with FLNA, preventing its proteolysis. When knock down, it prevents the interaction. Besides, it regulated by adhesion pathway and inhibited phosphorylation of FLNA by restrained PAK2, then, facilitated its proteolysis and its distribution to the nucleus, thereby suppression metastasis.