Fig 1.

The impact of ENST00000489676 overexpression on proliferation, cell cycle and apoptotic ability of KTC1 cells.(A) qRT-PCR analysis of the expression level of ENST00000489676 in KTC1 and TPC1 cells. (B) qRT-PCR analysis of the expression level of ENST00000489676 in KTC1 cells infected with Lenti-vector and Lenti-ENST00000489676. The values were normalized to GAPDH mRNA expression. (C) CCK-8 assay was performed to determine the KTC1 cell proliferation ability after 24hours, 48hours, 72 hours and 96 hours with ENST00000489676 overexpressed. (D)Colony formation assay was performed to determine the proliferation ability of KTC1 cells infected with Lenti-vector and Lenti-ENST00000489676. The colonies were captured and counted. The number of colonies was presented in histogram. (E) Flow cytometry images of cell cycle in KTC1 cells infected with Lenti-vector and Lenti-ENST00000489676. Results quantified in cell cycle were showed as a percentage of total cells. (F) Flow cytometry images of cell apoptosis in KTC1 cells infected with Lenti-vector and Lenti-ENST00000489676. Results quantified in viable cells were showed as a percentage of total cells. All data were expressed as mean ±SD of three independent experiments (“*” indicates P<0.05, “***” indicates P<0.001).