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. 2019 Aug 29;10(22):5434–5446. doi: 10.7150/jca.31751

Fig 3.

Fig 3

The impact of ENST00000489676 knockdown on proliferation, cell cycle and apoptotic ability of KTC1 cell.(A) qRT-PCR analysis of the expression level of ENST00000489676 in KTC1 cells infected with pLKO-NC, pLKO-shRNA1 and pLKO-shRNA2. The values were normalized to GAPDH mRNA expression. (B) CCK-8 assay was performed to determine the KTC1 cell proliferation ability after 24hours, 48hours, 72 hours and 96 hours with ENST00000489676 knocked down. (C)Colony formation assay was performed to determine the proliferation ability of KTC1 cells infected with pLKO-NC, pLKO-shRNA1 and pLKO-shRNA2. The colonies were captured and counted. The number of colonies was presented in histogram. (D) Flow cytometry images of cell cycle in KTC1 cells infected with pLKO-NC, pLKO-shRNA1 and pLKO-shRNA2. Results quantified in cell cycle were showed as a percentage of total cells. (E) Flow cytometry images of cell apoptosis in KTC1 cells infected with pLKO-NC, pLKO-shRNA1 and pLKO-shRNA2. Results quantified in viable cells were showed as a percentage of total cells. All data were expressed as mean ±SD of three independent experiments (“*” indicates P<0.05, “**” indicates P<0.01, “***” indicates P<0.001).