Fig 6.

The impact of ENST00000489676 knockdown and overexpression on migration and invasion of TPC1 cell. Wound healing assay was performed to determine the migration ability of TPC1 cells with ENST00000489676 knocked down (A) and overexpressed (B). Representative images of 0 hour and 24 hours of three repeated experiments were showed. Transwell assay was performed to determine the invasion ability of TPC1 cells with ENST00000489676 knocked down (C) and overexpressed (D). The representative images of invaded cells at lower chamber were stained with crystal violet. The quantifications of cell invasion were presented as invaded cell numbers. All data were expressed as mean±SD of three independent experiments (“**” indicates P<0.01, “***” indicates P<0.001).