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. 2019 Aug 29;10(22):5434–5446. doi: 10.7150/jca.31751

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Fig 8 — ENST00000489676 knockdown and overexpression regulate the impact of TPC1 cell on endothelial cell migration and invasion. HUVECs were incubated with the supernatant of TPC1 cells transfected with pLKO-NC, pLKO-shRNA1, pLKO-shRNA2, Lenti-vector and Lenti-ENST00000489676 respectively. Then HUVECs were subjected to wound healing assay and matrigel invasion assay. ENST00000489676 knockdown and overexpression in TPC1 cells affect the migration (A) (B) and invasion (C) (D) ability of endothelial cells. Representative micrographs of HUVECs stimulated with supernatants of ENST00000489676 knocked down and overexpressed TPC1 cells were shown. All data were expressed as mean±SD of three independent experiments. ( “***” indicates P<0.001).