Fig 9.

ENST00000489676 regulates KTC1 and TPC1 cell invasion by negatively modulating miR-922. (A)The bioinformatic analysis under MIRDB4.0(http://mirdb.org/miRDB/index.html) predicted a target site on ENST00000489676 that was complementary with miR-922;(B) qRT-PCR analysis of the expression level of miR-922 in KTC1 or TPC1 cells infected with Lenti-vector, Lenti-ENST00000489676, pLKO-NC, pLKO-shRNA1 and pLKO-shRNA2 respectively. (C) (E) Transwell assay was performed to determine the invasion ability of KTC1 cells under co-transfection of Lenti-ENST00000489676 and miR-922 inhibitors. (D) (E) Transwell assay was performed to determine the invasion ability of TPC1 cells under co-transfection of Lenti-ENST00000489676 and miR-922 inhibitors. The representative images of invaded cells at lower chamber were stained with crystal violet. The quantifications of cell invasion were presented as invaded cell numbers. All data were expressed as mean±SD of three independent experiments ( “*” indicates P<0.05 ,“**” indicates P<0.01, “***” indicates P<0.001).