GC patients had increased levels of CD68+ macrophages in the peritoneum, rather than the tumor bed and surgical margin. A. Representative pictures of immunohistochemistry staining of CD68 (stained in brown) in the specimens (400×). The numbers of CD68+ cells in the primary tumor bed (B), surgical margin (C) showed no statistical significance between the GC patients without peritoneal metastasis and those with peritoneal metastasis. In GC patients with peritoneal metastasis, peritoneal metastatic lesions had a similar number of CD68+ cells compared to the adjacent peritoneal tissue (D and E). F. GC patients with peritoneal metastasis harbored more CD68+ cells in the Pouch of Douglas with respect to GC patients without peritoneal metastasis. GC: gastric cancer. PM: peritoneal metastasis. n.s., no significance; *, p<0.05, by student t tests.