Expression of CD34 in the primary GC tumor bed, surgical margin and pouch of Douglas. No difference in the expression of CD34 on the primary tumor bed was observed between the non-metastasis and metastasis group. The expressions of CD34 at the surgical margins and in the Douglas' Pouch in metastasis group were higher than those of non-metastasis group, respectively. A. The expression of CD34 at different sites (400×). B, C, and D, quantification of CD34 expression in different sites. B. tumor bed; C. Surgical margin; D. Douglas' Pouch. PM: peritoneal metastasis. n.s., no significance; *, p<0.05; ***, p<0.001, by student t tests.