Validation of transfection efficiency of mimic or antagomir on miR-378a-3p expression. (A) Relative expression of miR-378a-3p in U87MG and MT-330 cells treated with miR-378a-3p mimic or antagomir. The relative expression levels of miR-378a-3p were normalized with the matched NC. (B) Relative expression of TSPAN17 mRNA in U87MG and MT-330 cells treated with both miR-378a-3p antagomir and si-TSPAN17. (C) Relative expression of TSPAN17 protein in U87MG and MT-330 cells treated with both miR-378a-3p antagomir and si-TSPAN17. Data are presented as the mean ± standard deviation. All the data are representative of five independent experiments (n=5). miR, microRNA; mimic, miR-378a-3p mimic; mimic-NC, miR-378a-3p mimic negative control; antagomir, miR-378a-3p antagomir; anta-NC, miR-378a-3p antagomir negative control; si-NC, siRNA negative control; si-TSPAN17, TSPAN17 siRNA; anta + si, miR-378a-3p antagomir + TSPAN17 siRNA.