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. 2019 Aug 23;42(5):1781–1792. doi: 10.3892/or.2019.7293

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Copyright: © Zhuang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Identification of TGF-β1 as a modulator of radiation-induced fibroblast proliferation and FMT. (A) Heat map of RNAs differentially regulated by radiation in fibroblasts. ‘Red’ indicates upregulation, and ‘blue’ indicates downregulation. (B) RT-qPCR validation of differentially regulated RNAs in fibroblasts, fibroblasts treated with radiation, and fibroblasts co-cultured with MSCs^Hypoxia or MSCs^Hypoxia + anti-HGF antibody in the presence of radiation. ^*P<0.05 vs. the Control; ^▲P<0.05 vs. Radiation + MSCs^Hypoxia. In fibroblasts, fibroblasts treated with radiation, and fibroblasts co-cultured with MSCs^Hypoxia or MSCs^Hypoxia + recombinant TGF-β1 in the presence of radiation, (C) proliferation growth curves were determined using an MTT assay. (D and E) Col1 and α-SMA mRNA levels as analyzed by RT-qPCR. Each column represents the mean ± SD from three independent experiments; ^*P<0.05 vs. the Control; ^▲P<0.05 vs. Radiation + MSCs^Hypoxia. FMT, fibroblast-to-myofibroblast transition; MSCs, mesenchymal stem cells; HGF, hepatocyte growth factor; Col1, type I collagen; α-SMA, α-smooth muscle actin.

Figure 3. — Identification of TGF-β1 as a modulator of radiation-induced fibroblast proliferation and FMT. (A) Heat map of RNAs differentially regulated by radiation in fibroblasts. ‘Red’ indicates upregulation, and ‘blue’ indicates downregulation. (B) RT-qPCR validation of differentially regulated RNAs in fibroblasts, fibroblasts treated with radiation, and fibroblasts co-cultured with MSCs^Hypoxia or MSCs^Hypoxia + anti-HGF antibody in the presence of radiation. ^*P<0.05 vs. the Control; ^▲P<0.05 vs. Radiation + MSCs^Hypoxia. In fibroblasts, fibroblasts treated with radiation, and fibroblasts co-cultured with MSCs^Hypoxia or MSCs^Hypoxia + recombinant TGF-β1 in the presence of radiation, (C) proliferation growth curves were determined using an MTT assay. (D and E) Col1 and α-SMA mRNA levels as analyzed by RT-qPCR. Each column represents the mean ± SD from three independent experiments; ^*P<0.05 vs. the Control; ^▲P<0.05 vs. Radiation + MSCs^Hypoxia. FMT, fibroblast-to-myofibroblast transition; MSCs, mesenchymal stem cells; HGF, hepatocyte growth factor; Col1, type I collagen; α-SMA, α-smooth muscle actin.