Figure 3.

Effects of silymarin on apoptotic cell in AGS human gastric cancer cells. (A) AGS cells were treated with silymarin for 24 h and apoptotic bodies stained with DAPI. The arrows indicate chromatin condensation in the AGS cells. Fragmentation of nuclear DNA was examined by a fluorescence microscope (×200). Arrows indicate apoptotic bodies, which represent DNA fragments produced during apoptosis. (B) AGS cells were treated with silymarin for 24 h and nuclear condensation was examined by DAPI staining. Graphs reveal quantification of DNA fragmentation and nuclear condensation. Each bar represents the mean ± SD calculated from five independent experiments. *P<0.05, statistically significant compared with untreated controls (Dunnett's t-test).