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. 2019 Sep 12;42(5):1815–1824. doi: 10.3892/or.2019.7309

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — The growth-promoting effect of Rap1A in ESCC is context-dependent. (A and B) The Rap1A mRNA or protein expression level in ECA109 cells transfected with p-shRap1A1, p-shRap1A2, p-shRap1A3, p-shRap1A4 and the p-control. (C) RT-qPCR and western blot analysis results of Rap1A knockdown efficiency following transfection with lentiviral vectors containing one of the two most efficient shRap1A or LV-NC in ECA109 (upper panel) and KYSE150 (lower panel) cell lines. (D) Cell viability, determined by an MTT assay, as described in the methods. (E) The colony-forming ability of the indicated cells was measured using a colony formation assay after incubation for 15 days; error bars represent the mean ± standard error of the mean; **P<0.01, ***P<0.001. Rap1A, Ras-associated protein 1A; ESCC, esophageal squamous cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.