Fig. 4.

Effects of carbon sources and amino acids on the growth of the acI strains IMCC25003 (figures on the left: a, c, e, and g) and IMCC26103 (figures on the right: b, d, f, and h). a and b Effects of amino acid mixture (AA) and/or carbon source mixture (CM). AA and CM were added to the FAMV medium separately or together. Detailed medium composition is presented in Table S1. c and d Effects of individual carbon compound. Each of 5 carbon compounds [5 μM, except for pyruvate (50 μM)] was added to the FAMV+AA medium. All, 5 carbon substrates were added together; None, no carbon source was added. e and f Effects of individual amino acids. Each of 20 proteinogenic amino acids was added at a concentration of 100 nM to the basal medium FAMV. All, 20 amino acids were added together; None, no amino acid was added. g and h Optimal concentrations of l-methionine (for IMCC25003) and l-cysteine (for IMCC26103). Various concentrations (0–10,000 nM) of l-methionine or l-cysteine were added to the basal medium FAMV. All experiments were performed in triplicate. Error bars indicate standard deviation. Note that error bars shorter than the size of the symbols are hidden