Fig. 5.

Adiposity-associated microorganisms. a Overlap analysis of the shared individuals in both the abdominal fat weight (AFW) and abdominal fat percentage (AFP) ranked groups of the highest 20% (N = 40) and lowest 20% (N = 40), respectively. b Significant p-values for each Wilcoxon rank-sum test (see methods). Displayed from the outer to the inner circle are the gut segment, the significance test in each microbial abundance between the highest- and lowest-AFP birds (p_AFP–m), the significance test in each microbial abundance between the highest- and lowest-AFW birds (p_AFW–m), the significance test in AFP between the two groups with the highest and lowest microbial abundance (p_m–AFP), and the significance test in AFW between the two groups with the highest and lowest microbial abundance (p_m–AFW), where p-values are plotted as −log₂ (p-value); the blue dashed line shows the significance threshold (p < 0.05). Each point represents a microorganism and the red point indicates the p-value passed the significance threshold. The grey dashed line indicates that the p_AFW–m, p_m–AFW, p_AFP–m and p_m–AFP values for one microorganism are all < 0.05. c The number of microbial genera associated with AFP and AFW at p < 0.05 and their overlaps with each other. The 12 shared microbial genera are considered potentially related to fat deposition. d Pearson’s (upper diagonal) and Spearman’s (lower diagonal) correlations among the AFW, AFP and the shared 12 microbial genera. Red and blue tiles indicate positive and negative correlations, respectively; significant R-values are filled in numerically (p < 0.05). The diagonal shows the fatness traits or gut segments (C caecum, F faeces, I ileum, J jejunum)