Fig. 2.

The community changed morphology frequency of autotorphic and diazotrophic cells as members were recruited during succession. a Confocal micrographs showing changes in biofilm community morphology as visualized by the DNA stain (Hoechst; blue) and chlorophyll autofluorescence (red); scale bars represent 20 µm. b Magnitude and distribution of ¹³C and ¹⁵N incorporation in representative biofilm communities showing incorporation of labelled substrates over different incubation periods. Top row: δ¹³C distribution after incubation with ¹³C-labelled bicarbonate. Bottom row: δ¹⁵N distribution after incubation with ¹⁵N-labelled N₂. The full suite of replicate nanoSIMS images are presented in a supplementary file