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PR-619 induces histone H2AX phosphorylation. (A and B) K562 cells were treated with etoposide (50 µM) or PR-619 (80 µM) for 2 hours, fixed with paraformaldehyde, and TOP2A or TOP2B and γH2AX were detected by immunofluorescences. Images are representative examples from three replica experiments. (C) H2AX phosphorylation per nucleus was assessed by quantitative immunofluorescent. Data represent mean ± S.D., individual data points are indicated as blue-lined circles; scale bar, 10 μm.
