Fig. 4.

Bayesian phylogenomic tree of representative Rickettsiales and other Alphaproteobacteria. Inference was based on the concatenated protein alignment (34,747 characters) from 120 highly conserved orthologs [36] with the LG+I+G+F evolutionary model. Maximum-likelihood bootstrap values (with 1000 pseudo-replicates) and posterior probabilities (after 100,000 generations) of internal tree nodes are shown on branches (fully supported values of 100|1.00 were omitted). The newly characterised Deianiraea putative parasite of P. primaurelia CyL4-1 is evidenced in bold. The representatives of each of the four families of Rickettsiales (Rickettsiaceae, Anaplasmataceae, Midichloriaceae, and Deianiraeaceae) are evidenced in red on the right side. The three clades including other 49 total Alphaproteobacteria (two clades) and the outgroup (composed by 17 other Proteobacteria), are shown collapsed as trapezoidal shapes (full trees are shown in Supplementary material 24c, d). The scale bar stands for estimated 0.1 sequence divergence